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SKKB3121 BIOPROCESS ENGINEERING LABORATORY 1

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Topic outline

  • General

    skkb3121
    Lecturer : Mr. Nik Azmi Bin Nik Mahmood
    Prof. Dr. Rosli Md illias
    Prof. Dr. Ida Idayu Muhammad
    Mr. Mohd Hafzan Saidin
    Mr. Nur Muhammad ElQarni Md Norudin
    Mr.Yaakop Sabuddin


    Semester :  1 Session 2015/2016

    Synopsis :

    This laboratory course is designed to expose students to conduct basics microbiology and biochemistry. The students will be subjected to handling bacterial culture, and  analysis of biomolecule such as enzyme, DNA, proteins and carbohydrate.

    Learning Outcomes:

    1. Explain and demonstrate simple techniques for handling bacterial culture such as its growth control, aseptic culturing techniques, staining and microscopic observation.
    2. Analyze and evaluate biochemical analysis on biomolecules such as DNA, protein and carbohydrate
    3. Effectively work in a team to accomplish the experiment.
    4. Effectively apply good and safe practice in laboratory.

    Creative Commons License This work, SKKB3121 Bioprocess Engineering Lanoratory 1 by Mr. Nik Azmi Bin Nik Mahmood is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License
  • Topic 1

    Experiment 1:

    Basic Microbiological Techniques For Isolation, Cultivation and Culture Characterization
  • Topic 2

    Experiment 2:

    Microscopic Analysis Of Microorganism
  • Topic 3

    Experiment 3:

    Sterilization and Microbial Growth Control


  • Topic 4

    Experiment 4:

    Cellulose Degradation and Glucose Analysis
  • Topic 5

    Experiment 5:

    Kinetics Analysis of Amyloglucosidase Hydrolysis of Soluble Starch
  • Topic 6

    Experiment 6:

    Protein Extraction and Protein Analysis using Biuret  Method 
  • Topic 7

    Experiment 7:

    DNA Extraction and DNA Analysis Using Eletrophoresis
  • Topic 8

    Open Ended Experiment


  • Topic 9

    Safety Video Assignment